A Polyacrylamide Gel Electrophoretic Approach of Fingerprinting Soil Polyphenols
نویسندگان
چکیده
منابع مشابه
Obtaining replicas from a polyacrylamide gel at different stages of the same electrophoretic run.
Varying the duration of electrophoresis produces different patterns in the same gel at the same power settings. However, with the present methods for visualizing proteins, only one electrophoretic separation can be obtained from one electrophoretic experiment. Thus, only a part of each electrophoretic experiment’s analytical potential is usually exploited. This is particularly evident in isoele...
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Solubility and stability properties of almond proteins were determined using ultracentrifugation and gel electrophoresis to gain a better insight into the complexity of these proteins. Ultracentrifugal analyses of the water-extractable proteins of defatted almond meal revealed four fractions of 2S, 9S, 14S and 19S. The 14S fraction corresponds to amandin, the classical globulin isolated earlier...
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2-Oxoisovalerate palmitoylcarnitine and 2-oxoisovalerate were 1 .O, 0.3 and 0.07 respectively. The rate of oxidation of 2-oxoisovalerate determined spectrophotometrically was about 12 times faster than when measured at 30°C by "CO, release from 2-0xo[ 114C]isovalerate by rat liver mitochondria1 fractions (Bremer & Davis, 1978; Patel et al., 1980). a-Cyano-4-hydroxycinnamate specifically inhibit...
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Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.
متن کاملIsolation and properties of the electrophoretic components of human growth hormone by Sephadex-gel filtration and preparative polyacrylamide-gel electrophoresis.
1. Human growth hormone was prepared from acetone-dried residues after extraction of gonadotrophins from pituitary glands. 2. Crude growth hormone was purified by gel filtration on Sephadex, resulting in a product that is soluble in water or 0.5% sodium chloride. It is painless on injection and shows a twofold increase in biological potency. Aggregation of growth hormone on Sephadex columns can...
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ژورنال
عنوان ژورنال: International Journal of Soil Science
سال: 2005
ISSN: 1816-4978
DOI: 10.3923/ijss.2006.53.57